Construction and production of Lentiviral Vectors

Lentiviral vectors are derived from the human immunodeficiency virus (HIV). They have the capacity to integrate their genome into a cell (max 8kb) but have been relieved of their replicative capacity. As a result, the DNA integrated into the cell will be transmitted during cell division to daughter cells without producing viral particles.

Production is carried out by transient transfection of helper plasmids and the plasmid containing your transgene into human cells. Viral particles will be produced by the use of cellular machinery and recovered by ultracentrifugation.
You can entrust us with the construction of your plasmid-transgene, or give us directly your already constructed plasmid.

3 production sizes are available:

  • mini production: around 50µL of viral vector;
  • maximum production: approximately 150 to 200 μL of viral vector;
  • gigabyte production: around 300 to 400µL of viral vector.

3 types of titrations are available:

  • titration in ELISA of detection of the capsid protein P24 (quantification of the quantity of physical particles);
  • titration by qPCR after infection of cells (Quantification of the number of viral particles capable of infecting a cell);
  • titration by flow cytometry if presence of a fluorochrome (Quantification of the number of infected cells capable of expressing the integrated transgene).